Protein Solubility Calculator

Chemistry

Calculate protein solubility in solution. Converts between concentration units: g/L, mg/mL, molarity (M). Affected by pH, temperature, salt concentration, and protein type.

Formula

Solubility = Mass (g) ÷ Volume (L)
  • Concentration = Mass ÷ Volume
  • Molarity = (Mass ÷ Molar Mass) ÷ Volume
  • Protein solubility depends on: pH (isoelectric point), temperature, ionic strength (salting out/in)
  • Most proteins soluble at neutral pH in aqueous solutions
  • Some proteins precipitate at high salt concentrations (salting out)

Example

Inputs
  • Protein Mass (g): 10
  • Solution Volume (L): 1
  • Molar Mass (g/mol): 50000

10 g of protein (MW 50 kDa) in 1 L: Concentration = 10 g/L ≈ 0.0002 M (200 µM).

Frequently asked questions

What factors affect protein solubility?
pH (isoelectric point), temperature, salt concentration, presence of organic solvents, and protein hydrophobicity.
What's molar mass?
Molecular weight in g/mol. For proteins, typically 5,000-500,000 g/mol depending on size.